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Rule out the usual hiding places first. If the piece really is gone, check the set piece by piece and leave with the element ID LEGO asks for, or a link to buy the part.
Builders who report a missing piece often find it in one of these places. Tick each one off as you look.
LEGO's request form asks for the set number (10543) and each piece's element ID, both listed above.
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Duplo Car Base 2 x 4 with Fixed Axles - 27.5 Yellow Wheels and Black Tires
×1
Duplo Door / Window Frame Flat Front Surface, Completely Open Back
×1
Duplo Plate 2 x 4
×1
Duplo Brick 1 x 2 x 2
×2
Duplo Brick 2 x 4
×1
Duplo Brick 1 x 2 x 2
×2
Duplo Plate 2 x 4
×1
Duplo Plate 2 x 8
×1
Duplo Brick 2 x 4 x 2 Arch
×1
Duplo Brick 2 x 4 Curved Top
×2
Duplo Cape with Elastic Loop
×1
Duplo Fire Hydrant
×1
Duplo Brick 1 x 2 x 2 with Daily Planet Logo Print
×1
Duplo Door / Window with Four Equal Square Cornered Panes
×1
Duplo Car Body with 2 Studs on Back and Silver Headlights Print
×1
Duplo Figure with Parted Wavy Hair Black, with Light Nougat Face (Superman)
×1
Tick each piece as you find it, or mark it missing.
LEGO takes requests for pieces missing from a new set through its customer service site. The form asks for the set number and each piece's element ID, the number printed beside the piece in the parts list at the back of the instructions. This checker lists both.
Request a replacement from LEGOMark a piece missing and your list links it to an eBay search for that part, which also covers pieces from older or second-hand sets.
Copy or download your missing list (the CSV opens in any spreadsheet), or print the whole checklist and tick pieces off on paper. Your marks stay in this browser, so you can check a big set over several sittings.
